Pandu jaet com4/11/2023 ![]() ![]() litura shifted into the artificial diet using 3-5 replicates. The suspension treated for 20 individuals of first instar larvae S. The formulation was exposed to sunlight for 0, 1, 2, and 3 weeks. thuringiensis serotype kurstaki strain HD-7 applied from commercial product DiPel-WP®. This research formulated 2.5% of the original substance of A. atlas (L.) cocoon extract as UV protectant B. The objective of this research was to determine the effectiveness of adding A. thuringiensis has disadvantage of being easily degraded due to sunlight. to enhance its persistence for future formulations.īacillus thuringiensis serotype kurstaki is an entomopathogenic bacteria commonly used to control the cutworm Spodoptera litura (Fab.). Based on this research, it can be concluded that the addition of eri and atlas silk moths sericin extracts show high potential for the UV B protectants for Bt. toxicity by 1.75 – 1.86 and 1.6 – 2.1 fold, respectively, even after four weeks of UV B exposure. The pathogenicity results implied that the addition of eri and atlas silk moths cocoon extracts significantly improved the Bt. Whereas, at the 4th week, the mortality of each treatment was 46.67, > 81, and > 75% respectively. At the 1st week of UV B exposure, the mortality of tobacco cutworm in Bt. were significant difference compared to Bt. The results showed that the mortality percentage of tobacco cutworm treated with cocoon added Bt. Then, the pathogenicity was tested against 25 individuals of 1st larval instar of tobacco cutworm using three replicates. After that, the suspensions then exposed under UV B lights at laboratory conditions for 1, 2, 3, and 4 weeks. suspensions at 10LC95 (2 × 108 spores/ml). Cocoon extracts of 0, 0.5, 1, 2, and 2.5% of eri and atlas sericin was added respectively to make Bt. ![]() against the larval stage of tobacco cutworm. (Lepidoptera: Saturniidae) cocoon extracts as UV B protectants for Bt. This study aimed to determine the effectiveness of eri, Samia ricini Drury (Lepidoptera: Saturniidae) and atlas moth, Attacus atlas L. However, this bioinsecticide might easily degraded by ultraviolet (UV) from the sun light. kurstaki (Bt.) is a bioinsecticide that is often used as an alternative for controlling tobacco cutworm, Spodoptera litura Fab. ![]()
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